Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: Microarray Analyses (A) for 5 Pairs of Retinoblastoma Tissues and Para‐carcinoma Tissues, and Expressions (B) of 3 Mostly Over‐expressed and Under‐expressed lncRNAs were Confirmed within the Included Retinoblastoma and Para‐carcinoma Samples. *: P < 0.05 when compared with adjacent non‐tumour tissues

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Microarray

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: The Expressions of LncRNA HOTAIR and miR‐613 within Retinoblastoma Tissues and Cells. A, HOTAIR and miR‐613 expressions were compared between retinoblastoma tissues and adjacent non‐tumour tissues. *: P < 0.05 when compared with adjacent non‐tumour tissues. B, HOTAIR and miR‐613 expressions were compared among ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1 cell lines. *: P < 0.05 when compared with ACBRI‐181 cell line. C, The HOTAIR expression was negatively correlated with the miR‐613 expression within retinoblastoma tissues. D, The survival rates of retinoblastoma patients with up‐ and down‐regulated HOTAIR expressions were compared. E, The survival rates of retinoblastoma patients with highly and lowly expressed miR‐613 were compared

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: The correlation between main characteristics and the retinoblastoma patients’ overall survival

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: After Transfection of pcDNA3.1‐HOTAIR, si‐HOTAIR, miR‐613 mimic and miR‐613 Inhibitor into Retinoblastoma Cells (A), the Results of Colony CCK‐8 Assay (B) and Formation Assay (C) were Evaluated among the NC, si‐HOTAIR, pcDNA3.1‐HOTAIR, miR‐613 mimic and miR‐613 Inhibitor Groups. *: P < 0.05 when compared with NC

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Transfection, CCK-8 Assay, Tube Formation Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: The Correlation Between miR‐613 and c‐met. A, HOTAIR expression was positively correlated with c‐met expression within retinoblastoma tissues. B, MiR‐613 expression was negatively correlated with HOTAIR expression within retinoblastoma tissues. C, The c‐met expression within Y79 and HXO‐Rb44 cell lines was compared among si‐HOTAIR, pcDNA3.1‐HOTAIR, miR‐613 inhibitor, miR‐613 mimic and NC groups. *: P < 0.05 when compared with NC. D, MiR‐613 was targeted by certain sites within c‐met. E, The luciferase activities of Y79 and HXO‐Rb44 cell lines were compared between miR‐613 mimic + c‐met Wt and miR‐613 mimic + c‐met Mut groups. *: P < 0.05 when compared with miR‐NC

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Expressing, Luciferase

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: After Transfection of pcDNA3.1/c‐met into Retinoblastoma Cells (A), CCK‐8 Assay (B) and Colony Formation Assay (C) were Conducted to Compare the Proliferative Statuses of Y79 and HXO‐Rb44 Cell Lines among miR‐NC, miR‐613 mimic and miR‐NC + c‐met Groups. *: P < 0.05 when compared with miR‐NC or NC

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Transfection, CCK-8 Assay, Colony Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA HOTAIR/miR‐613/c‐met axis modulated epithelial‐mesenchymal transition of retinoblastoma cells

doi: 10.1111/jcmm.13796

Figure Lengend Snippet: The relationship between lncRNA HOTAIR/microRNA‐613 expressions and the retinoblastoma patients’ main characteristics

Article Snippet: Human retinoblastoma cell lines were purchased from American Type Culture Collection (ATCC), including ACBRI‐181, Y79, HXO‐Rb44, SO‐Rb50 and WERI‐RB1.

Techniques: Expressing

Journal: BMC Biotechnology

Article Title: Simultaneous silencing of multiple RB and p53 pathway members induces cell cycle reentry in intact human pancreatic islets

doi: 10.1186/1472-6750-14-86

Figure Lengend Snippet: Simultaneous knockdown of multiple genes in primary human islets following electroporation. (A) Graph shows RT-qPCR results of islets electroporated with non-targeting control siRNA or siRNA targeting RB and p130 (L-003299). (B) Graph shows RT-qPCR results of islets electroporated with non-targeted control siRNA or siRNAs targeting the indicated gene products. The housekeeping gene GAPDH was used as the control. Representative experiment shown from 3 separate experiments. Error bars indicate standard deviation. (C) Western blot for RB protein in human islet lysates after electroporation of control or targeting siRNA. GAPDH was used as a loading control. RB appears as a characteristic doublet representing the hypo and hyperphosphorylated protein. Representative experiment shown from 3 different experiments.

Article Snippet: For detection of Retinoblastoma protein blots were probed with 0.1 μg/mL of Human RB1 (MAB6495, R&D Systems) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (R &D Systems).

Techniques: Electroporation, Quantitative RT-PCR, Standard Deviation, Western Blot

Journal: Cell Reports Medicine

Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

doi: 10.1016/j.xcrm.2025.101966

Figure Lengend Snippet:

Article Snippet: pCMV3-His-Rb , Sino Biological , HG10137-NH.

Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software

Journal: JNCI Journal of the National Cancer Institute

Article Title: ARRB1-Mediated Regulation of E2F Target Genes in Nicotine-Induced Growth of Lung Tumors

doi: 10.1093/jnci/djq541

Figure Lengend Snippet: Effect of nicotine on association between E2F1 and ARRB1 in human non–small cell lung cancer cells. A) Serum-starved A549 were treated with 1 μM nicotine for 15 minutes, and localization of ARRB1 and E2F1 was analyzed by double-immunoflourescence staining followed by confocal microscopy (×630 magnification, scale bar = 5 μm). The cells were counterstained with the nuclear marker, 4′6-diamidino-2-phenylindole. Overlay of the images show yellow spots indicating colocalization in the bottom right panel. B) Effect of overexpression of pcDNA3-FLAG-rat ARRB1 (wild type) and pcDNA3-HA-E2F1 in HEK293 cells. HEK293 cells were transfected with the above plasmids, and the physical interaction between ARRB1 and E2F1 was analyzed 24 hours after transfection by immunoprecipitation with mouse anti-HA antibody followed by immunoblotting using mouse anti-FLAG antibody. In addition, immunoblot analysis was done for FLAG and HA expression. C) Real-time kinetics of ARRB1–E2F1 interaction upon nicotine treatment of A549 cells. Serum-starved A549 cells were treated with 1 μM nicotine for 15 minutes, 30 minutes, and 1 hour, and the physical interaction between ARRB1 and E2F1 was analyzed by immunoprecipitation–immunoblot assay. Furthermore, the E2F1 immunoprecipitates were immunoblotted for RB1 to demonstrate equivalent amount of E2F1. D) Effect of nicotine on the association of ARRB1 and E2F1 in normal lung epithelial cells, NHBEs and SAECs. Quiescent NHBEs and SAECs were treated with 1 μM nicotine for 15 minutes, and the physical interaction between ARRB1 and E2F1 was analyzed by immunoprecipitation–immunoblot analysis. Furthermore, the E2F1 and ARRB1 immunoprecipitates were immunoblotted with E2F1 and ARRB1 to demonstrate equivalent amounts of protein. E) Effect of nicotine on binding of ARRB1 to E2F1, RB1 and EP300. Serum-starved A549 cells were treated with 1 μM nicotine for 2 hours. The binding of ARRB1 to E2F1, EP300, and RB1 was analyzed by immunoprecipitation–immunoblotting experiment. Immunoprecipitation with anti-mouse HA antibody was used as the negative control for the experiment. The above-mentioned immunoprecipitates were immunoblotted for RB1 protein. All the immunofluorescence and immunoprecipitation–immunoblotting experiments are representative of two independent experiments. IB = immunoblot; IP = immunoprecipitation.

Article Snippet: Mouse anti-human RB1 was purchased from BD Biosciences (San Jose, CA).

Techniques: Staining, Confocal Microscopy, Marker, Over Expression, Transfection, Immunoprecipitation, Western Blot, Expressing, Binding Assay, Negative Control, Immunofluorescence

Journal: JNCI Journal of the National Cancer Institute

Article Title: ARRB1-Mediated Regulation of E2F Target Genes in Nicotine-Induced Growth of Lung Tumors

doi: 10.1093/jnci/djq541

Figure Lengend Snippet: The levels of ARRB1–E2F1 complexes and association of E2F1, ARRB1, Ac-H3, and EP300 with BIRC5 promoter in human NSCLC tumors. A) The levels of ARRB1–E2F1 complexes in human NSCLC tumor tissues relative to matched distant normal lung tissues. Tissue lysates were prepared from human NSCLC tumors (n = 8) and matched distant normal lung tissue (n = 8). Immunoprecipitation–immunoblotting analysis was performed to compare the levels of ARRB1–E2F1 complexes in the above lysates. The E2F1 immunoprecipitates were analyzed for the level of RB1 protein by immunoblotting. The NSCLC tumor tissues were also analyzed for levels of E2F1, ARRB1, RB1, and α7-nAChR relative to matched distant normal lung tissue. ACTB was used as a loading control. B) Relative association of E2F1, ARRB1, Ac-H3, and EP300 with BIRC5 promoter in human NSCLC tumor tissue relative to matched distant normal lung tissue. Chromatin immunoprecipitation (ChIP) assays were performed to compare the levels of E2F1, ARRB1, Ac-H3, and EP300 bound to BIRC5 promoter in these tissues. FOS promoter was used as a negative control. Rabbit anti-mouse IgG was used as the irrelevant antibody for all ChIP reactions. The input DNA lane represents one-fifth of the precleared chromatin used in each ChIP reaction. The results presented in this figure are representative of two independent immunoprecipitation–immunoblotting assays. Ab = antibody; IB = immunoblot; IP = immunoprecipitation.

Article Snippet: Mouse anti-human RB1 was purchased from BD Biosciences (San Jose, CA).

Techniques: Immunoprecipitation, Western Blot, Chromatin Immunoprecipitation, Negative Control